Genethon’s Imaging Cytometry platform (ImCy), with over 15 years’ experience in supporting researchers in gene therapy, is not only an expert in acquisition of cytometry data and microscopy images, but also in deep and quantitative analysis of these data and images.
Introduction
The presence within the same department of cytometry and imaging experts provides mutual enrichment between experts from these two fields.
We have a very rich instrument base and we offer our services to Genethon’s research teams, and also in a collaborative or service formation, to the teams from the Biotherapies Institute at AFM-Telethon as well as to private or public members of the Genopole biocluster (Evry-Courcouronnes), or national and international parties interested in our renowned expertise.
For any requests, please contact the Imaging platform.
The members of the platform
Activities
Genethon’s Imaging Cytometry platform (ImCy) has a variety of activities based mainly on data acquisition and analysis.
We offer image acquisition, with different degrees of definition and resolution, on our different machines, independently and assisted, as well as image analysis using different commercial solutions or solutions developed by us. We offer acquisition of cytometry data, cellular sorting and analysis of these data using different software programs adapted to the devices and needs of our customers.
We also provide our partners with:
- Our consulting expertise for preparing or designing a project or experiment
- Our digital modeling expertise
- Our cross-disciplinary expertise for creating collaborative connections with other platforms or research teams (atomic force microscopy, biophysical analysis methods, NMR, etc.).
Our experience in the field of imaging, cytometry, genetic diseases and biotherapies makes our platform a renowned center in the Paris region, particularly for analysis of the efficacy of gene therapies applied to muscle cells.
Beyond our rejuvenation policy, offering the most advanced technologies possible, our platform is a national center for imaging and cytometry applied to quantification of the effects of therapies on rare diseases, and in particular neuromuscular diseases, strengthening and exchanging our know-how through many collaborations.
Equipment
We own an equipment base with complementary imaging and cytometry machines, which allows analysis of biological samples at different scales. Our aim is to support integrative and multimodal studies from analysis of whole small animals via bioluminescence to location of proteins in super resolution (STED), and phenotyping of cell populations using cytometry.
Along with our machines, we own independent analysis stations, some of them equipped with software specific to certain analysis (Imaris, etc.).
Imaging
| CELLULAR IMAGING | ||
|---|---|---|
| Name | Supplier | Description |
| Axioscan Z1 [in French] | Zeiss | Blade scanner (100 blade loader) 7-color LED lamp |
| Confocal TCS SP8 [in French] | Leica | Confocal microscope with resonant scanner and Gated STED module 775nm |
| α3 [in French] | PhaseView | Light sheet microscope 4 lasers |
| Evos FL | Life Technologies | Inverted fluorescence microscope for cell culture 3 colors |
| Biostation IM | Nikon | Light field and fluorescence time-lapse microscope (2 channels) |
| HoloMoniror M4 | PHI Imaging | Holographic time-lapse microscope |
| AxioPlan FL | Zeiss | Straight fluorescence microscope + QImaging Retiga 2000R Camera |
| BF morphometry | Nikon | Light field straight microscope for mapping |
| FL morphometry | Leica | Straight fluorescence microscopy for mapping 3 colors + polarization |
| XF | Nanolive | Tomography microscope holographic time-lapse |
| Eclipse Ti Inverted FL | Nikon | Inverted fluorescence microscope 3 colors + Hamamatsu Orca-spark monochrome camera |
| SMALL ANIMAL IMAGING | ||
|---|---|---|
| Name | Supplier | Description |
| IVIS Lumina | Perkin Elmer | Small animal bioluminescence imaging |
| CELLVIZIO | Mauna Kea | Confocal micro-endoscope through fiber-optic bundle dedicated to small animals |
Cytométrie
| CONVENTIONAL SPECTRAL – IMAGING | ||
|---|---|---|
| Name | Supplier | Description |
| MoFlo ASTRIOS EQ [in French] | Beckman-Coulter | High-throughtput cell sorter 4 Lasers 14 Param. Fluo. + Morpho |
| CytoFLEX LX [in French] | Beckman-Coulter | Flow cytometer with plates and microtubes loader 6 Lasers 21 Param. Fluo. + Morpho |
| CytoFLEX S [in French] | Beckman-Coulter | Flow cytometer with plate loader 4 Lasers 13 Param. Fluo. + Morpho |
| SP6800 [in French] | Sony Biotechnology Inc. | Spectral flow cytometer 3 Lasers 32 PMTs |
| ISX MKII [in French] | CYTEK | Image flow cytometer 3 Lasers 6 channels |
Operations
Our operations are based on a clearly established and transparent relationship between our customers and ourselves by the signature of a user charter.
Indeed each user of Genethon’s Imaging Cytometry platform must sign the OCCIGEN user charter [in French] which establishes the platform’s commitment criteria – safety information, supply of calibrated equipment, performance of services and analysis within a pre-set deadline, provision of technical support, respect of confidentiality, etc. – and the customer’s commitment criteria – observance of safety and machine usage instructions, information on the nature of samples, payment for services rendered, mention of the platform in all communications, etc.
We also offer training on our equipment, allowing you to use our platform completely independently when the project requires.
For requests for access to equipment, services, training, information or price list, please contact us.
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